12 string acoustic comparable?

I'm sure that it is just fine e for a 12 string. I can't say for sure though.

Is this tuner actually "chromatic" will it tune a string to A# or Gb? and will it tune lower than 440hz?

Yes. The chromatic mode does work.

Will this tuner work on a nylon string guitar?

Yes it picks up by vibration. Only old 2 AA battery tuners that plugged in viavguitarcvordcwere restricted to sound only using a condesir onboard mic or using guitar cord . All you clip on tuners work vibration so it works in noisy places without disturbing the musician from tuning you be good. Notevthewe are simple tuners using setting for alternitivevtunjings you may want a shark tuner . These are great for 440 tuning or getting use to in E Flat

Package Dimensions	10.8 x 4.9 x 3.7 cm; 60 g
Batteries	2 CR2 batteries required. (included)
Body Material	Lithium
Material Type	Plastic, Rubber/Silicone
Item Weight	60 g

Guitar tuner 2 PACK, Clip On Tuner for Guitar/Bass/Violin/ukulele,Auto Power Off/One Button Operation/AT-01A/2 Set

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