The AzuraQuantTM HRM Fast Mix is a ready-to-use 2x master mix for use in high-throughput, fast high-resolution melt (HRM) for the analysis of mutations, polymorphisms and epigenetic differences in double-stranded DNA. The system contains Azura HS Taq polymerase, high affinity antibodies and Vivid-GreenTM 2 dye, a novel saturating, third-generation dye which is ideal for accurate SNP genotyping. The AzuraQuantTM HRM Fast Mix has been developed and optimized for fast PCR cycling, improved sensitivity, and maximum discrimination between sequence variants. The latest advances in enzyme preparation coupled with the optimization of ionic conditions and enhancers enable the amplification and clear discrimination of even the most challenging sequence variations including Class IV SNP (Single Nucleotide Polymorphisms) without sequence preference.
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